Andreia S. Azevedo

Junior Researcher

Research group: Biotechnology

Background: PhD in Chemical and Biological Engineering – Faculty of Engineering of Porto, Portugal

Major research topics

• Multispecies Biofilms
• Development of nucleic acid mimics-based methods for microbial detection by Fluorescent in situ hybridization (NAM-FISH)
• Combinatorial labelling and spectral imaging - fluorescence in situ hybridization for the study of microbiotas
• Understanding the mechanistic of NAM-FISH

Selected Publications

Azevedo AS, Sousa IM, Fernandes RM, Azevedo NF, Almeida C. Optimizing locked nucleic acid/2’-O-methyl-RNA fluorescence in situ hybridization (LNA/2’OMe-FISH) procedure for bacterial detection. PLoS ONE 2019 14(5): e0217689.

Azevedo AS, Almeida C, Melo LF, Azevedo NF. Impact of polymicrobial biofilms in catheter-associated urinary tract infections. Crit Rev Microbiol. 2017 Aug;43(4):423-439.

Azevedo AS, Almeida C, Gomes LC, Ferreira C, Mergulhão FJ, Melo LF, Azevedo NF. An in vitro model of catheter associated urinary tract infections to investigate the role of uncommon bacteria on the Escherichia coli microbial consortium. Biochem Eng J. 2017 Feb; 118:64-69.

Azevedo AS, Almeida C, Pereira B, Melo LF, Azevedo NF. Impact of Delftia tsuruhatensis and Achromobacter xylosoxidans on Escherichia coli dual-species biofilms treated with antibiotic agents. Biofouling. 2016 Mar;32(3):227-41.

Azevedo AS, Almeida C, Pereira B, Madureira P, Wengel J, Azevedo NF. Detection and discrimination of biofilm populations using Locked nucleic acid/2'-O-methyl- RNA fluorescence in situ hybridization (LNA/2'OMe-FISH). Biochem Eng J. 2015 Dec; 104:64-73.

Selected Projects

Development of NAM-CLASI-FIVH method: a new in vitro tool for an accurate analysis of diversity and spatial organization of gastric microbiota (Ongoing), Project Reference: POCI-01-0145-FEDER-030431, Funding entity: Portuguese Foundation for Science and Technology (FCT), Duration: 2018 - 2021, Role: Principal Investigator

De novo selection of toxin-specific aptamers using nucleic acid mimics (Ongoing), Project Reference: POCI-01-0145-FEDER-028659, Funding entity: Portuguese Foundation for Science and Technology (FCT), Duration: 2018 - 2021, Role: Team member

Development of a novel colour-coded RING FISH method: an innovative tool for the detection of antibiotic resistant clinical pathogens (Ongoing), Project Reference: POCI-01-0145-FEDER-016678, Funding entity: Portuguese Foundation for Science and Technology (FCT), Duration: 2016 - 2019, Role: Team member

DNA mimics: Nucleic acid mimics for the rapid detection of microorganisms, Project Reference: PIC/IC/82815/2007, Funding entity: Portuguese Foundation for Science and Technology (FCT), Duration: 2012 - 2015, Role: Team member