Email:

asazevedofe.up.pt

Phone: +351 22 041 3603

Room: E307

Laboratory: E007

Junior Researcher

Research group: Biotechnology

Background: PhD in Chemical and Biological Engineering – Faculty of Engineering of Porto, Portugal

Andreia S. Azevedo

Major research topics

• Multispecies biofilms
• Development of nucleic acid mimic-fluorescent in situ hybridization (NAM-FISH)
• Spectral imaging and Confocal laser scanning microscopy
• Multiplexed fluorescence in situ hybridization for spatial location of biofilms

Selected Publications

Azevedo AS, Fernandes RM, Faria AR, Silvestre OF, Nieder JB, Lou C, Wengel J, Almeida C, Azevedo NF. Spectral imaging and nucleic acid mimics fluorescence in situ hybridization (SI-NAM-FISH) for multiplex detection of clinical pathogens. Front Microbiol. 2022 Sep 29;13:976639.

Barbosa A, Miranda S, Azeevdo NF, cerqueira L. Imaging biofilms using fluorescence in situ hybridization: seeing is believing. Front Cell Infect Microbiol (2023), 13.

Allkja J, Goeres DM, Azevedo AS, Azevedo NF. Interactions of microorganisms within a urinary catheter polymicrobial biofilm model. Biotechnol Bioeng. 2023 Jan;120(1):239-249.

Nácher-Vázquez M, Barbosa A, Armelim I, Azevedo AS, Almeida GN, Pizarro C, Azevedo NF, Almeida C, Cerqueira L. Development of a Novel Peptide Nucleic Acid Probe for the Detection of Legionella spp. in Water Samples. Microorganisms. 2022 Jul 13;10(7):1409.

Azevedo AS, Sousa IM, Fernandes RM, Azevedo NF, Almeida C. Optimizing locked nucleic acid/2’-O-methyl-RNA fluorescence in situ hybridization (LNA/2’OMe-FISH) procedure for bacterial detection. PLoS ONE 2019 14(5): e0217689.

Selected Projects

Development of NAM-CLASI-FIVH method: a new in vitro tool for an accurate analysis of diversity and spatial organization of gastric microbiota (Ongoing), Project Reference: POCI-01-0145-FEDER-030431, Funding entity: Portuguese Foundation for Science and Technology (FCT), Duration: 2018 - 2021, Role: Principal Investigator

NAMSal - Nucleic acid mimics as a new tool against salmonellosis in the poultry industry (Ongoing), Project Reference: 2022.07654.PTDC, Funding entity: Portuguese Foundation for Science and Technology (FCT), Duration: 2023 - 2026, Role: Team member.

LegioFilms - Understanding the Role of Biofilm Architecture in Legionella Colonization and Risk of Detachment in hospital networks using an Integrated Monitoring Approach (Ongoing), Project Reference: 2022.03523.PTDC, Funding entity: Portuguese Foundation for Science and Technology (FCT), Duration: 2023 - 2026, Role: Team member.