25 years of Science and Innovation




Phone: +351 22 041 4945

Room: INESC 4.9

Laboratory: E007

Junior Researcher

Background: PhD in Chemical and Biological Engineering - University of Minho, Portugal

Nuno Guimarães

Major research topics

  • Fluorescence in situ Hybridization
  • Nucleic Acid Mimics
  • Target delivery systems
  • Antisense therapeutics

Selected Publications

Moreira, L., N. M. Guimaraes, S. Pereira, R. S. Santos, J. A. Loureiro, R. M. Ferreira, C. Figueiredo, M. C. Pereira and N. F. Azevedo. "Engineered liposomes to deliver nucleic acid mimics in Escherichia coli." J Control Release (2023) 355: 489-500.

Moreira, L., N. M. Guimaraes, S. Pereira, R. S. Santos, J. A. Loureiro, M. C. Pereira and N. F. Azevedo. "Liposome Delivery of Nucleic Acids in Bacteria: Toward In Vivo Labeling of Human Microbiota." ACS Infect Dis (2022) 8(7): 1218-1230.

Pereira, S., R. S. Santos, L. Moreira, N. Guimaraes, M. Gomes, H. Zhang, K. Remaut, K. Braeckmans, S. De Smedt and N. F. Azevedo. "Lipoplexes to Deliver Oligonucleotides in Gram-Positive and Gram-Negative Bacteria: Towards Treatment of Blood Infections." Pharmaceutics (2021) 13(7).

Santos RS, Lima CC, Carvalho D, Meireles F, Guimarães N, Azevedo NF. "Response surface methodology to optimize peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) in Saccharomyces cerevisiae." LWT - Food Science and Technology (2017) 80:27-31.

Fontenete S, Carvalho D, Guimaraes N, Madureira P, Figueiredo C, Wengel J, et al. "Application of locked nucleic acid-based probes in fluorescence in situ hybridization." Appl Microbiol Biotechnol (2016) 100(13):5897-906.

Selected Projects

NAMSal - Mímicos de ácidos nucleicos como uma nova ferramenta para o combate de salmonelose na indústria pecuária- 2022.07654.PTDC. Duration 2023 – 2026; Role: Team member

DelNAM - Strengthening the Research Area of Delivery of Nucleic acid mimics (NAMS) into bacteria to fight the antibiotic crisis - WIDESPREAD-05-2017. Duration 2018-2021; Role: Team member.

uFISH - Integração de hibridação in situ fluorescente com microfluídica para deteção rápida de patogéneos alimentares - POCI-01-0145-FEDER-031011. Duration: 2018 - 2021; Role: Team member

ColorISH - Development of a new colorimetric method for the rapid detection of Legionella spp. in water sources - POCI-01-0145-FEDER-29961. Duration: 2018 - 2021Role: Team member

Coded-FISH - Development of a novel color-coded RING FISH method: an innovative tool for the detection of antibiotic resistant clinical pathogens - PTDC/DTP-PIC/4562/2014|16678. Duration: 2016 - 2019; Role: Team member


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