Nuno F. Azevedo

Senior Researcher

Research group: Biotechnology

Academic position: Associate Professor (FEUP)

Background: PhD in Chemical and Microbiological Technology – University of Minho, Portugal

Major research topics

• Multispecies Biofilms.
• Development of hybridization-based techniques.
• Nucleic acid mimics for therapy and diagnostics.
• Bioinformatics.
• Mechanistics of biotechnological processes.

Selected Publications

Moreira L., Guimaraes N.M., Pereira S., Santos R.S., Loureiro J.A., Ferreira R.M., Figueiredo C., Pereira M.C. and N.F. Azevedo “Engineered liposomes to deliver nucleic acid mimics in Escherichia coli”. Journal of Controlled Release (2023), 355:489-500.

Azevedo A.S., Fernandes R.M., Faria A.R., Silvestre O.F., Nieder J.B, Lou C., Wengel J., Almeida C. and N.F. Azevedo “Spectral imaging and nucleic acid mimics fluorescence in situ hybridization (SI-NAM-FISH) for multiplex detection of clinical pathogens”. Frontiers in Microbiology (2022), 13:976639.

Moreira L., Guimaraes N.M., Pereira S., Santos R.S., Loureiro J.A., Pereira M.C. and N.F. Azevedo “Liposomes delivery of nucleic acids in bacteria: Towards in vivo labelling of human microbiota”. ACS Infectious Diseases (2022), 8(7):1218–1230.

Oliveira R., Pinho E., Sousa A.L., DeStefano J.J., Azevedo N.F. and C. Almeida “Improving Aptamer Performance with Nucleic Acid Mimics: De Novo and Post-SELEX approaches”. Trends in Biotechnology (2022), 40(5):549-563.

Azevedo N.F., Allkja J. and D.M. Goeres “Biofilms vs. cities and humans vs. aliens – a tale of reproducibility in biofilms”. Trends in Microbiology (2021), 29(12): 1062-1071.

Selected Projects

“e.BIOFILM - Creation of a group of Excellence on Engineered Biofilms” (ref. 101087568), funded by H2020 – European Commission. Beginning: March 2023. Duration: 48 months. Role: Co-Principal Investigator.

“NAMSal - Nucleic acid mimics as a new tool against salmonellosis in the poultry industry" (ref. 2022.07654.PTDC), funded by FCT/FEDER. Beginning: March 2023. Duration: 36 months. Role: Principal Investigator.

"DelNAM - Strengthening the Research Area of Delivery of Nucleic Acid Mimics (NAMS) into bacteria to fight the antibiotic crisis", funded by European Commission (Horizon 2020). Starting Date: 2018; Duration 36 months. Role: Principal Investigator.

“µFISH - Integration of fluorescence in situ hybridization with microfluidics for the quasi real-time detection of food pathogens", funded by Fundação para a Ciência e Tecnologia. Starting Date: 2018; Duration 36 months. Role: Principal Investigator.

“PRINT-AID: anti-infective medical devices combining printing technologies and antimicrobial functionality”, an European Training Network funded by the European Commission. Starting Date: 2017; Duration 36 months. Role: Principal Investigator for FEUP’s Team.